Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for doxorubicin delivery to colorectal cancer cells

Y Yan; APR Johnston; SJ Dodds; MMJ Kamphuis; C Ferguson; RG Parton; EC Nice; JK Heath; F Caruso

Journal article

Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for doxorubicin delivery to colorectal cancer cells

Y Yan, APR Johnston, SJ Dodds, MMJ Kamphuis, C Ferguson, RG Parton, EC Nice, JK Heath, F Caruso

ACS Nano | Published : 2010

DOI: 10.1021/nn100173h

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Abstract

Understanding the interactions between drug carriers and cells is of importance to enhance the delivery of therapeutics. The release of therapeutics into different intracellular environments, such as the lysosomes or the cell cytoplasm, will impact their pharmacological activity. Herein, we investigate the intracellular fate of layer-by-layer (LbL)-assembled, submicrometer-sized polymer hydrogel capsules in a human colon cancer derived cell line, LIM1899. The cellular uptake of the disulfide-stabilized poly(methacrylic acid) (PMA SH) capsules by colon cancer cells is a time-dependent process. Confocal laser scanning microscopy and transmission electron microscopy reveal that the internalized..

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University of Melbourne Researchers

Joan Heath Author

Frank Caruso Author

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Grants

Awarded by National Health and Medical Research Council (NHMRC)

Funding Acknowledgements

This work was supported by the Australian Research Council under the Federation Fellowship (F.C.) and Discovery Project (F.C.) schemes and by National Health and Medical Research Council (NHMRC) Project Grant 433613 (J.K.H., F.C., E.C.N.) and Program Grant 487922 F.C.). We gratefully acknowledge the assistance of Cameron Nowell (Centre for Advanced Microscopy, Ludwig Institute for Cancer Research, Melbourne) for helpful discussions regarding the confocal microscopy data.